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目的探讨伊贝沙坦对单侧输尿管梗阻大鼠模型肾间质纤维化的影响。方法制作大鼠肾间质纤维化模型,随机分为假手术组、模型组和伊贝沙坦治疗组,术后第7d、14d分别处死各组中8只大鼠,用免疫组化方法测定肾间质中血小板衍生生长因子(PDGF-BB)、血小板衍生生长因子受体(PDGFR-β)、α-平滑肌肌动蛋白(α-SMA)表达,观察肾脏病理变化。结果与模型组相比,治疗组肾间质纤维化明显减轻,肾间质中α-SMA、PDGF-BB、PDGFR-β表达明显减少(P<0.05)。结论伊贝沙坦可通过下调肾间质PDGF-BB、PDGFR-β表达,减轻肾间质纤维化。
Objective To investigate the effect of irbesartan on renal interstitial fibrosis in rats with unilateral ureteral obstruction. Methods A rat model of renal interstitial fibrosis was established and randomly divided into sham-operation group, model group and irbesartan-treated group. Eight rats in each group were sacrificed on the 7th day and the 14th day after operation, respectively. Immunohistochemistry The expressions of PDGF-BB, PDGFR-β and α-SMA in renal interstitium were observed. The pathological changes of renal were observed. Results Compared with model group, the expression of α-SMA, PDGF-BB and PDGFR-β in renal interstitium was significantly decreased (P <0.05). Conclusion Irbesartan can reduce renal interstitial fibrosis by down-regulating the expression of PDGF-BB and PDGFR-β in renal interstitium.