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应用染色体原位杂交、ＰＣＲ扩增、克隆及核苷酸序列分析等方法，分析了ＣＮＥ１和ＣＮＥ３细胞株中的潜伏感染膜蛋白（ＬＭＰ１）基因。ＣＮＥ１是来自我国东北的高分化鼻咽癌细胞株，ＣＮＥ３是来自广西的低分化鼻咽癌细胞株。染色体原位杂交结果表明，ＣＮＥ１细胞中ＬＭＰ１基因存在于细胞核内，整合在第一号染色体上，ＣＮＥ３中ＬＭＰ１基因则随机存在于细胞核内及多条染色体上。用ＰＣＲ方法分别从ＣＮＥ１及ＣＮＥ３中扩增得到了ＬＭＰ１基因片段（外显子３），核苷酸序列分析证明，来自ＣＮＥ１的ＬＭＰ１与来自Ｂ９５－８细胞的ＬＭＰ１核苷酸序列同源性极高，达９９．５％，而ＣＮＥ３的ＬＭＰ１基因与Ｂ９５－８的ＬＭＰ１基因同源性为９３％。
The latent membrane protein (LMP1) gene in CNE1 and CNE3 cell lines was analyzed by chromosomal in situ hybridization, PCR amplification, cloning and nucleotide sequence analysis. CNE1 is a well-differentiated nasopharyngeal carcinoma cell line from northeast China, and CNE3 is a poorly-differentiated nasopharyngeal carcinoma cell line from Guangxi. Chromosome in situ hybridization results showed that, LMP1 gene in CNE1 cells exist in the nucleus, integrated in the first chromosome, CNE3 LMP1 gene is randomly present in the nucleus and multiple chromosomes. The LMP1 gene fragment (exon 3) was amplified by PCR from CNE1 and CNE3, respectively. Nucleotide sequence analysis showed that LMP1 from CNE1 was homologous to the LMP1 nucleotide sequence from B95-8 cell High, up to 99.5%, whereas the homology of the LMP1 gene of CNE3 to that of B95-8 was 93%.