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目的和方法：在新生大鼠海马神经元上，用膜片钳技术研究了乙酰胆碱（ＡＣｈ）受体单通道特性、离子选择性及细胞内物质对通道活动的影响。结果：该通道对单价阳离子通透，对Ｎａ＋和Ｋ＋的通透性相近，但不通透Ｃｌ－。当膜片与细胞分离后，单个独立短开放通道的开放时间τ０１、τ０２和开放概率分别下降了６５．８％、５０．８％和６８．４％；对于长开放通道影响更为明显，开放时间τｂ１、τｂ２和开放概率分别下降了３２．３％、８５．９％和８４．６％。结论：胞内物质如第二信使、Ｃａ２＋等对海马神经元ｎＡＣｈＲ通道有调制作用
PURPOSE AND METHODS: Patch clamp technique was used to study the effects of ACh receptors’ single-channel properties, ion selectivity and intracellular substances on channel activity in neonatal rat hippocampal neurons. Results: The channel was permeable to monovalent cations with similar permeability to Na + and K +, but not to Cl-. When the membrane was separated from the cells, the open time τ01, τ02 and the open probability of a single independent short open channel decreased by 65.8%, 50.8% and 68.4%, respectively; the effect on the long open channel was more obvious and open Time τb1, τb2 and open probability decreased by 32.3%, 85.9% and 84.6% respectively. CONCLUSION: Intracellular substances such as second messenger, Ca2 + have modulatory effect on nAChR channel in hippocampal neurons