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目的:探讨原发性肝细胞癌(hepatocellular carcinoma,HCC)患者外周血单个核细胞(peripheral blood mononrclear cells,PBMCs)IL-22和IL-17 mRNA的表达水平及其临床意义。方法:收集2011年4月至10月暨南大学医学院附属深圳市人民医院感染内科经临床确诊为HCC患者40例,所有患者均为乙型肝炎表面抗原阳性。同时选取来自本科室健康人的外周静脉血20例作为对照。以常规淋巴细胞分层液密度梯度离心法分离得到PBMCs,采用RT-PCR法检测HCC患者组和健康人对照组PBMCs IL-22和IL-17 mRNA的表达水平。结果:与健康人对照组相比,HCC患者组IL-22 mRNA表达水平明显下降(0.309±0.044 vs 0.560±0.029,P<0.01),而IL-17 mRNA的表达水平则显著升高(0.682±0.048 vs 0.541±0.038,P<0.01)。结论:IL-17 mRNA的高表达可能参与HCC的发生、发展,而IL-22 mRNA低表达可能与HCC患者的近期预后不佳有关。
Objective: To investigate the expression of IL-22 and IL-17 mRNA in peripheral blood mononuclear cells (PBMCs) in patients with primary hepatocellular carcinoma (HCC) and its clinical significance. Methods: Forty patients with HCC diagnosed as clinically diagnosed as HCC from April 2011 to October 2011 in Shenzhen People’s Hospital of Jinan University were enrolled. All patients were positive for hepatitis B surface antigen. At the same time, 20 cases of peripheral venous blood from healthy people in our hospital were selected as control. The PBMCs were isolated by routine lymphocyte stratified liquid density gradient centrifugation, and the expression levels of IL-22 and IL-17 mRNA in PBMCs of HCC patients and healthy controls were detected by RT-PCR. Results: Compared with healthy controls, the expression of IL-22 mRNA was significantly decreased in HCC patients (0.309 ± 0.044 vs 0.560 ± 0.029, P <0.01), while the expression of IL-17 mRNA was significantly increased (0.682 ± 0.048 vs 0.541 ± 0.038, P <0.01). Conclusion: The high expression of IL-17 mRNA may be involved in the development of HCC. The low expression of IL-22 mRNA may be related to the poor prognosis of HCC patients.