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目的对昆明市流感监测地区的流感样病例咽拭子进行监测,以掌握2009～2010年昆明市甲型H1N1流感流行情况。方法提取标本中的病毒核酸,采用荧光定量PCR方法进行甲型H1N1分型。结果 1377份流感样标本中,检出A型流感病毒622份,甲型H1N1519份占总采样数的37.7%,以2009年10月～2010年1月为流行高峰,各年龄段人群均易感,30岁以下患者占84.4%。结论甲型H1N1在监测时间内,主要集中在2009年,流行季节为秋冬季,侵袭的人群以30岁以下年龄段为主。
Objective To monitor the throat swab of influenza-like illness in the area of influenza surveillance in Kunming in order to grasp the prevalence of influenza A (H1N1) in Kunming from 2009 to 2010. Methods The viral nucleic acids in samples were extracted and the type A H1N1 was typed by fluorescence quantitative PCR. Results Among the 1377 influenza samples, 622 influenza A viruses were detected and A H1N1519 accounted for 37.7% of the total number of samples, which was the peak of the epidemic from October 2009 to January 2010 and was susceptible to all age groups , 84.4% of patients under 30 years of age. Conclusion During the monitoring period, H1N1 mainly concentrated in 2009 and the epidemic season was autumn and winter. The affected population was mainly under the age of 30 years.