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对灯盏花遗传转化中的组织培养受体系统进行试验研究 ,发现以MS为基本培养基 ,使用BA0 2 +NAA2 0的激素配比及浓度 ,播种期 10d的幼苗 ,取真叶作为外植体 ,大小 0 5× 1 5cm2 ,能在二周内较快形成愈伤组织。使用MS +BA0 5+NAA0 5培养基、加 3 0 g/L的蔗糖 ,以真叶为外植体 ,能在二周内较快诱导不定芽形成。使用 75 μg/ml的chl作为土壤农杆菌转化培养的选择压力 ,使用 5 0 μg/ml的Gent作为转基因植株的选择压力 ,使用 3 0 0 μg/ml的Cef作为土壤农杆菌转基因的抑菌抗生素 ,对灯盏花遗传转化效果最佳
Tissue culture receptor system in the genetic transformation of Erigeron breviscapus was studied and found to use MS as a basic medium, the use of BA0 2 + NAA20 hormone ratio and concentration, sowing seedlings 10d, take the leaves as explants , The size of 0 5 × 1 5cm2, can quickly form callus within two weeks. Using MS + BA0 5 + NAA0 5 medium supplemented with 30 g / L sucrose and true leaves as explants, adventitious buds could be rapidly induced within two weeks. Using 75 μg / ml chl as selection pressure for Agrobacterium tumefaciens transformation culture, Gent at 50 μg / ml was used as selection pressure for transgenic plants, Cef at 300 μg / ml was used as antibacterial antibiotic for Agrobacterium tumefaciens transgene , Breviscapus genetic transformation best