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目的获取并分析志贺菌由抗生素诱导产生的耐多药相关基因序列。方法以志贺菌敏感株(Z23)和诱导株(YD)基因组DNA为模板,利用引物设计软件Primer 5.0根据已测得基因序列(Y16B3、Y16B8和Y16A11)设计引物,PCR扩增诱导耐多药相关基因并将相关基因克隆到pMD19-T载体上,测序并将结果在序列相似性搜索工具Blast上检索分析相关基因序列。结果Y16B3和Y16B8基因片段分别在诱导株中扩增出320和225 bp产物,在敏感株中未扩增出;而Y16A11基因片段在诱导株扩增出310 bp产物,在敏感株中扩增出554 bp产物,并且产物序列同源性极低。结论志贺菌在抗生素诱导下产生的耐多药株与敏感菌株比较,具有基因组差异。
Objective To acquire and analyze the multidrug-related gene sequence induced by antibiotics in Shigella. Methods Primer 5.0 was designed based on the sequenced gene sequences (Y16B3, Y16B8 and Y16A11) using primer design software Primer 5.0 and PCR amplification was performed to induce MDR The related genes were cloned into pMD19-T vector, sequenced and the results were searched on the sequence similarity search tool Blast to analyze the related gene sequences. Results The Y16B3 and Y16B8 gene fragments amplified 320 and 225 bp products respectively in the induced strains and not amplified in the sensitive strains. The Y16A11 gene fragment amplified 310 bp in the induced strains and amplified in the sensitive strains 554 bp products, and the product sequence homology is very low. Conclusion The multi-drug resistant strains induced by antibiotics induced by Shigella have genome differences compared with susceptible strains.