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本文选用小鼠足垫肿胀反应和DNA合成试验,研究了氯丁二烯对C_(57)BL/6N小鼠细胞免疫功能、胸腺及脾脏细胞的毒性作用;同时观察了氯丁二烯作业工人血清中IgG、IgM、IgA的含量及末梢血T细胞百分率的变化。结果表明,氯丁二烯以5mg/kg·d的剂量给小鼠染毒14天,可明显抑制足垫肿胀反应,降低胸腺细胞DNA的合成和增强脾脏细胞的DNA合成。氯丁二烯接触工人血清中IgG的含量较对照组显著降低,但IgM、IgA的含量无明显变化。末梢血T细胞百分率在接毒工人中亦明显低于对照组。并发现血清IgG含量和末梢血T细胞百分率的变化比常规肝功能检查敏感。
In this paper, mouse foot pad swelling reaction and DNA synthesis test were used to study the cytotoxicity of chloroprene to cellular immunity, thymus and spleen cells in C57BL / 6N mice. Meanwhile, Serum IgG, IgM, IgA content and peripheral blood T cell percentage changes. The results showed that chloroprene at a dose of 5mg / kg · d for 14 days in mice, can significantly inhibit the footpad swelling reaction, reducing thymocyte DNA synthesis and enhance DNA synthesis of spleen cells. The content of IgG in serum of chloroprene exposed workers was significantly lower than that of the control group, but the content of IgM and IgA did not change significantly. Peripheral blood T cell percentage was also significantly lower than the control group. And found that serum IgG levels and peripheral blood T cell percentage changes than conventional liver function tests sensitive.