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目的制备骨肉瘤U-2OS细胞NOD/SCID小鼠肺转移模型,探讨CIK/NK细胞对肺转移瘤的杀伤作用。方法 60只NOD/SCID小鼠随机分为3组(每组20只),A组:每只鼠经尾静脉接种U-2OS细胞2×106(0.2 ml)后24 h,输注CIK/NK细胞(2×107/0.2 ml);B组:每只鼠经尾静脉接种U-2OS细胞2×106(0.2 ml)后24 h,注射生理盐水0.2 ml;C组:每只鼠经尾静脉注射生理盐水0.2 ml后24 h,注射生理盐水0.2 ml。比较各组小鼠生存时间及肺部肿瘤负荷情况。结果 B组接种U-2OS细胞后29 d内小鼠全部死亡,其中16只肺部出现肉眼可见瘤块;A组接种U-2OS细胞后60 d内小鼠死亡8只,肺部均发现肉眼可见瘤块,余12只肺部均无瘤块。A组平均生存时间显著高于B组,差异有统计学意义(P<0.05),而与C组比较差异无统计学意义(P>0.05)。B组小鼠死亡时体重明显低于A组,而肿瘤细胞比例明显高于A组(P<0.05)。结论 CIK/NK细胞在NOD/SCID小鼠体内有肯定的抗骨肉瘤肺转移瘤效果。
Objective To prepare a lung metastasis model of osteosarcoma U-2OS cells in NOD / SCID mice and investigate the killing effect of CIK / NK cells on lung metastases. Methods Sixty NOD / SCID mice were randomly divided into three groups (n = 20). Group A: 24 h after inoculation of 2 × 106 U-2OS cells into tail vein of each rat, CIK / NK (2 × 107 / 0.2 ml); Group B: each mouse was inoculated with 2 × 106 (0.2 ml) U-2OS cells through the tail vein 24 h after injection of saline 0.2 ml; Group C: After injection of 0.2 ml saline for 24 h, 0.2 ml normal saline was injected. The survival time and lung tumor burden in each group were compared. Results All the mice died within 29 days after inoculation of U-2OS cells in Group B, and 16 of the lungs in Group B showed macroscopic tumor masses. In Group A, 8 mice died within 60 days after inoculation of U-2OS cells, Visible tumor blocks, more than 12 lung no tumor blocks. The average survival time in group A was significantly higher than that in group B, with a significant difference (P <0.05), but not statistically different from that in group C (P> 0.05). The body weight of mice in group B was significantly lower than that in group A, while the proportion of tumor cells in group B was significantly higher than that in group A (P <0.05). Conclusion CIK / NK cells have positive anti-osteosarcoma lung metastases in NOD / SCID mice.