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Background::The pathogenesis of osteosarcoma (OS) is still unclear, and it is still necessary to find new targets and drugs for anti-OS. This study aimed to investigate the role and mechanism of the anti-OS effects of miR-296-5p.Methods::We measured the expression of miR-296-5p in human OS cell lines and tissues. The effect of miR-296-5p and its target gene staphylococcal nuclease and tudor domain containing 1 on proliferation, migration, and invasion of human OS lines was examined. The Student’s n t test was used for statistical analysis.n Results::We found that microRNA (miR)-296-5p was significantly downregulated in OS cell lines and tissues (control n vs. OS, 1.802 ± 0.313 n vs. 0.618 ± 0.235, n t= 6.402, n P < 0.01). Overexpression of miR-296-5p suppressed proliferation, migration, and invasion of OA cells. n SND1 was identified as a target of miR-296-5p by bioinformatic analysis and dual-luciferase reporter assay. Overexpression of n SND1 abrogated the effects induced by miR-296-5p upregulation (miRNA-296-5p n vs. miRNA-296-5p + SND1, 0.294 ± 0.159 n vs. 2.300 ± 0.277, n t= 12.68, n P = 0.003).n Conclusion::Our study indicates that miR-296-5p may function as a tumor suppressor by targeting n SND1 in OS.n “,”Background::The pathogenesis of osteosarcoma (OS) is still unclear, and it is still necessary to find new targets and drugs for anti-OS. This study aimed to investigate the role and mechanism of the anti-OS effects of miR-296-5p.Methods::We measured the expression of miR-296-5p in human OS cell lines and tissues. The effect of miR-296-5p and its target gene staphylococcal nuclease and tudor domain containing 1 on proliferation, migration, and invasion of human OS lines was examined. The Student’s n t test was used for statistical analysis.n Results::We found that microRNA (miR)-296-5p was significantly downregulated in OS cell lines and tissues (control n vs. OS, 1.802 ± 0.313 n vs. 0.618 ± 0.235, n t= 6.402, n P < 0.01). Overexpression of miR-296-5p suppressed proliferation, migration, and invasion of OA cells. n SND1 was identified as a target of miR-296-5p by bioinformatic analysis and dual-luciferase reporter assay. Overexpression of n SND1 abrogated the effects induced by miR-296-5p upregulation (miRNA-296-5p n vs. miRNA-296-5p + SND1, 0.294 ± 0.159 n vs. 2.300 ± 0.277, n t= 12.68, n P = 0.003).n Conclusion::Our study indicates that miR-296-5p may function as a tumor suppressor by targeting n SND1 in OS.n