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本实验用免疫组化方法检测大鼠嗅球在不同年龄阶段的Fos表达.结果如下:胚鼠18天时,嗅球颗粒细胞层最先出现少许Fos阳性神经元.随后至P7天前Fos阳性神经元以缓慢速度逐渐增多.僧帽细胞层和小球层的Fos阳性神经元出现较颗粒细胞晚,僧帽细胞在P7天、小球旁细胞在P10天才出现Fos表达.从P14天以后这三层的Pos阳性神经元数有较明显的增多,到P30天达第一次高峰,P40天稍有下降,P50天颗粒细胞层的Fos阳性神经元数又有所上升,P60天达第二次高峰,但僧帽细胞层和小球层的Fos阳性神经元却逐渐减少.到成年期(3个月),颗粒细胞层仍有少量的Fos阳性神经元,但僧帽细胞层和小球层不再表达Fos.而在老年期(30个月),整个嗅球已没有Fos的表达.因此本结果提示:①嗅球出现FOS表达的顺序是:颗粒细胞--僧帽细胞—小球层细胞,而消失的顺序则相反,这提示分化越早的细胞其保持分化的能力越长,而分化越晚的细胞其保持分化的能力越短;②嗅球三细胞层出现较多的Fos阳性神经元是在P30天至P60天,提示大部分细胞的分化期主要集中在生后30～60天之间.
In this experiment, the expression of Fos in rat olfactory bulb at different age stages was detected by immunohistochemistry.The results are as follows: Fos-positive neurons first appeared in the olfactory bulb granular layer at 18 days, The slow rate gradually increased.Fos-positive neurons in the mitral layer and the parietal layer appeared later than the granulosa cells, and on the P7 day, the mitochondria showed Fos expression on the P10 genus.After P14, the three-layer The number of Pos positive neurons increased obviously, reaching the first peak on P30 day and decreasing slightly on P40 day. The number of Fos positive neurons on P50 day increased again, reaching the second peak on P60 day, But the number of Fos-positive neurons decreased gradually in the mitochondria layer and in the mitochondria layer.With adulthood (3 months), there were still a few Fos-positive neurons in the granular cell layer, but the mitochondria layer and the globule layer were no longer Expression of Fos in the elderly (30 months), the entire olfactory bulb has no Fos expression.Therefore, the results suggest that: (1) olfactory bulb FOS expression appears in the order: granulosa cells - mitral cells - small ball cells, and disappear This, on the contrary, suggests that the earlier the differentiated cells maintain their ability to differentiate The longer the force, and the late differentiation of cells to maintain their ability to differentiate the shorter; ② olfactory bulb three layers of cells appear more Fos-positive neurons in the P30 days to P60 days, suggesting that most of the cell differentiation mainly in the students After 30 to 60 days.