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目的:探讨原因不明复发性流产患者蜕膜组织中Th1/Th2比率与巨噬细胞B7-1、B7-2表达水平的相关性,分析蜕膜组织中共刺激分子B7-1、B7-2及Th1/Th2比率失衡在原因不明复发性流产发病中的作用。方法:用双荧光标记流式细胞技术检测原因不明复发性流产妇女(流产组)和正常妊娠妇女(对照组)的蜕膜组织中Th1/Th2比率和巨噬细胞B7-1、B7-2的表达水平。结果:流产组妇女蜕膜组织中Th1/Th2比率(20.41±3.32)、巨噬细胞B7-1的表达水平(21.57±1.70)%明显高于对照组(P<0.01);流产组妇女蜕膜组织中巨噬细胞B7-2的表达水平(27.82±2.28)%明显低于对照组(P<0.01);流产组妇女蜕膜组织中Th1/Th2比率与蜕膜组织中巨噬细胞B7-1的表达水平之间存在显著正相关(r=0.8663,P<0.01);流产组妇女蜕膜组织中Th1/Th2比率与蜕膜组织中巨噬细胞B7-2的表达水平之间存在显著负相关(r=-0.9016,P<0.01)。结论:原因不明复发性流产患者蜕膜组织中B7-1表达水平增高、B7-2表达水平降低与蜕膜组织中Th1/Th2比率异常有密切的关系,可能是导致原因不明复发性流产发生的重要因素。
Objective: To investigate the correlation between the Th1 / Th2 ratio in decidual tissue and the expression of B7-1 and B7-2 in patients with unexplained recurrent spontaneous abortion, and to analyze the expression of costimulatory molecules B7-1, B7-2 and Th1 / Th2 imbalance in unexplained recurrent spontaneous abortion. Methods: The ratio of Th1 / Th2 in the decidual tissue of the unexplained recurrent spontaneous abortion (abortion group) and the normal pregnant women (control group) and the ratio of Th1 / Th2 in the macrophages B7-1 and B7-2 were determined by double fluorescent labeling flow cytometry The expression level. Results: The ratio of Th1 / Th2 (20.41 ± 3.32), B7-1 (21.57 ± 1.70)% in abortion group was significantly higher than that in control group (P <0.01) The expression level of B7-2 in macrophages was significantly lower than that in control group (27.82 ± 2.28)% (P <0.01). The ratio of Th1 / Th2 in decidua tissue of women with abortion was significantly lower than that in macrophages B7-1 (R = 0.8663, P <0.01). There was a significant negative correlation between Th1 / Th2 ratio in decidua and B7-2 level in macrophages in decidua (r = -0.9016, P <0.01). Conclusion: The expression of B7-1 in decidual tissue of patients with recurrent spontaneous abortion of unknown origin is increased. The decreased expression of B7-2 is closely related to the abnormal Th1 / Th2 ratio in decidual tissue, which may be the result of unexplained recurrent spontaneous abortion Key factor.