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为了建立非1B/1R类型K型小麦细胞质雄性不育系的分子标记辅助选育技术体系,对基础遗传材料Tm3314来自莫迦小麦1BS染色体的T型恢复基因Rf3和K型不育基因rfv1进行了分子标记定位。以Tm3314和T型不育系T504A的杂交F2代作为定位群体,利用分离集团分析法(BSA),从1BS染色体上10对SSR引物中筛选出与目的基因连锁的2个SSR标记Xbarc8和Xgwm18。然后结合(T504A/Tm3314)F2群体在T型细胞质下的育性分离情况和F2可育株与K型不育系K119A测交所得的K型细胞质下的育性结果,运用Mapmaker 3.0b软件进行连锁分析,结果表明,Xbarc8和Xgwm18与Rf3基因的遗传距离分别为5.5 cM和8.1 cM,与rfv1基因的遗传距离分别为22.2 cM和19.6 cM,且2个SSR标记位于两个育性基因之间。
In order to establish a molecular marker-assisted breeding technology system for non-1B / 1R K-type wheat cytoplasmic male sterile line, the Tm3314 T-type restorer gene and the K-type sterility gene rfv1 from the chromosome 1BS of Mozambique wheat were analyzed. Mark positioning. Two F2 generation SSR markers Xbarc8 and Xgwm18 linked to the target gene were screened out from 10 pairs of SSR primers on 1BS chromosome by using segregation group analysis (BSA) with the F2 generation of Tm3314 and T type sterile line T504A as the locating group. Then, the fertility of F2 population (T504A / Tm3314) in T-type cytoplasm and the fertility of K-type cytoplasm obtained by F2 fertile and K-type male sterile line K119A were determined by Mapmaker 3.0b software The results showed that the genetic distances between Xbarc8, Xgwm18 and Rf3 were 5.5 cM and 8.1 cM, respectively. The genetic distance to rfv1 was 22.2 cM and 19.6 cM respectively, and the two SSR markers were located between the two fertile genes .