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目的:探讨黄芪对慢性心衰大鼠心功能及心肌肌浆网钙泵基因(SERCA2a)表达的影响。方法:60只大鼠随机分为假手术组、模型组、黄芪组(20 g/kg)和卡托普利组(0.05 g/kg)。采用缩窄腹主动脉法制备慢性心衰模型,造模后第13周开始灌胃给药,连续给药12周后测定大鼠在体血流动力学指标,并采用RT-PCR、Western blot方法检测左室组织SERCA2a基因的表达。结果:模型大鼠左室收缩压(LVSP)、左室舒张末压(LVEDP)均较假手术组显著升高(P<0.01或P<0.001),卡托普利和黄芪均能降低模型大鼠LVSP(P<0.05或P<0.01)、LVEDP(P<0.05或P<0.01);模型组大鼠左心室SERCA2a基因表达较假手术组下调(P<0.05),黄芪能上调模型大鼠SER-CA2a基因表达(P<0.05),而卡托普利对其表达无明显影响。结论:黄芪能改善慢性心衰大鼠心功能障碍,并以改善舒张功能为主,其机制可能与上调心肌SERCA2a基因表达有关。
Objective: To investigate the effects of astragalus on heart function and expression of sarcoplasmic reticulum calcium pump gene (SERCA2a) in rats with chronic heart failure. Methods: Sixty rats were randomly divided into sham operation group, model group, Huangqi group (20 g/kg) and captopril group (0.05 g/kg). The chronic heart failure model was established by coarctation of abdominal aorta. At the 13th week after model establishment, intragastric administration was started. After 12 weeks of continuous administration, the hemodynamic parameters of rats were measured, and RT-PCR and Western blot were used. Methods The expression of SERCA2a gene in left ventricular tissue was detected. RESULTS: Left ventricular systolic pressure (LVSP) and left ventricular end-diastolic pressure (LVEDP) in model rats were significantly higher than those in the sham operation group (P<0.01 or P<0.001). Both captopril and jaundice reduced the rats’ rats. LVSP (P<0.05 or P<0.01), LVEDP (P<0.05 or P<0.01); Left ventricular SERCA2a gene expression in the model group was lower than that in the sham group (P<0.05). Huangqi could upregulate the SER- CA2a gene expression (P<0.05), while captopril had no significant effect on its expression. Conclusion: Astragalus membranaceus can improve cardiac dysfunction in rats with chronic heart failure and improve diastolic function. The mechanism may be related to up-regulation of SERCA2a gene expression in myocardium.