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自1969年Rygaard首次将人体结肠癌移植至裸小鼠获得成功后,在肿瘤的基础和临床研究中,裸小鼠已被广泛地应用。裸鼠是T细胞免疫缺陷型动物,故要在净化环境下才能保证正常繁育和动物实验,常用的净化饲养设备有无菌隔离器、净化空气室和层流架等。其中层流架具有价格低、操作简便、对外环境无特殊要求等优点。为了探讨层流架的实用价值,我们对ZPCJ-1型层流架进行了生物学检测及移植性肿瘤实验的研究。材料与方法 1.ZPCJ-1型双面正压层流架;系浙江省平阳交通净化设备厂产品。安置环境:具有双层玻璃窗的12m~2小间内,装有小型换气扇排气通风,外有4m~2
Since the first successful Rygaard transplant of human colon cancer to nude mice in 1969, nude mice have been widely used in basic and clinical studies of tumors. Nude mice are T cell immunodeficient animals, so in order to ensure normal breeding and animal experiments in the purification environment, commonly used purification equipment has a sterile isolator, clean air chamber and laminar flow rack and so on. Among them, the laminar flow frame has the advantages of low price, easy operation and no special requirement for the external environment. In order to explore the practical value of LHD, we carried out biological testing and transplantation tumor research on ZPCJ-1 LHD. Materials and methods 1.ZPCJ-1 double-sided positive pressure laminar flow frame; Department of traffic in Pingyang, Zhejiang Province purification plant products. Setting environment: with double glazing 12m ~ 2 small room, equipped with a small ventilator exhaust ventilation, outside 4m ~ 2