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本文用等电聚焦和聚丙烯酰胺凝胶电泳方法,对9个恶性疟原虫样品的磷酸葡萄糖异构酶(GPI)进行了观察。研究所用的恶性疟原虫得自亚洲、非洲和拉丁美洲。按Trager烛缸法培养。取感染和未感染的RBC收集在pH8.0的磷酸缓冲液中,1000g离心5分钟,弃上清,沉淀再重复一次,第二次沉淀物按1:9V/V悬浮于等电聚焦(IEF)缓冲液,制成的疟原虫样品做等电聚焦时,再次用IEF缓冲液稀释(1:1V/V),取20μl加至含有两性电解质(pH
In this paper, the phosphoglucose isomerase (GPI) of nine samples of Plasmodium falciparum was observed by isoelectric focusing and polyacrylamide gel electrophoresis. The Plasmodium falciparum used in the study was from Asia, Africa and Latin America. By Trager candle cylinder training. Infected and uninfected RBCs were harvested in phosphate buffered saline (pH 8.0), centrifuged at 1000g for 5 minutes, the supernatant was discarded and the pellet was resuspended once more. The second pellet was resuspended at 1: 9 V / V in isoelectric focusing (IEF ) Buffer, the plasmodium sample prepared was subjected to isoelectric focusing, diluted again with IEF buffer (1: 1 V / V), and 20 μl was added to a solution containing amphoteric electrolytes