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AIM: To study the association between APOE polymorphisms and cerebral infarction through a case-control study among the Chinese Han population. METHODS: First-ever cerebral infarction patients (n=226) whose ages ranged from 40 to 60 years old were recruited from Department of Neurology, Zhongshan Hospital, Shanghai, and Zhejiang Chinese Traditional Medicine Hospital, Zhejiang, China. Unrelated healthy controls (n=201) were selected from the general population in the same area with similar age and sex distribution. APOE was amplified by one-stage PCR using the forward primer: 5’-GGC ACG GCT GTC CAA GGA GCT-3’ and reverse primer: 5’-GAT GGC GCTGAG GCC GCG CT-3’. The PCR product was digested directly with 5 U of CfoI and separated by a 20 % polyacryla- mide (acrylamide: bis-acrylamide=29:1) nondenaturing gel. RESULTS: Both cerebral infarction patient and control groups were in Hardy-Weinberg equilibrium. The allele frequency of APOE*2, APOE*3, and APOE*4 was 4.6 %, 81.9 %, and 13.5 % respectively in the patients with cerebral infarction; 5.7 %, 87.3 %, and 7.0 % respectively in the healthy control group. Compared with APOE3/3 subjects, APOE4/4 carriers had a 2.1-fold risk of cerebral infarction (odds ratio 2.1, 95 % confidence limits 1.3 to 3.4). The allele frequency of APOE*4 in the cerebral infarction patient group was significantly higher than that in the control group (13.5 % vs 7.0 %; P=0.002). CONCLUSION: APOE 4 is a risk factor for cerebral infarction among the Chinese Han population.
AIM: To study the association between APOE polymorphisms and cerebral infarction through a case-control study among the Chinese Han population. METHODS: First-ever cerebral infarction patients (n = 226) aged ages range from 40 to 60 years old were were recruited from Department of Neurology, Zhongshan Hospital, Shanghai, and Zhejiang Chinese Traditional Medicine Hospital, Zhejiang, China. Unrelated healthy controls (n = 201) were selected from the general population in the same area with similar age and sex distribution. stage PCR using the forward primer: 5’-GGC ACG GCT GTC CAA GGA GCT-3 ’and reverse primer: 5’-GAT GGC GCTGAG GCC GCG CT-3’. The PCR product was digested directly with 5 U of CfoI and separated RESULTS: Both cerebral infarction patient and control groups were in Hardy-Weinberg equilibrium. The allele frequency of APOE * 2, APOE * 3, and APOE * 4 was 4.6%, 81.9%, and 13.5% resp Compared with APOE3 / 3 subjects, APOE4 / 4 carriers had a 2.1-fold risk of cerebral infarction (odds ratio 2.1, 95 % confidence limits 1.3 to 3.4). The allele frequency of APOE * 4 in the cerebral infarction patient group was significantly higher than that in the control group (13.5% vs 7.0%; P = 0.002). CONCLUSION: APOE 4 is a risk factor for cerebral infarction among the Chinese Han population.