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为研究不同初始C/N值的混合物料对甘蔗叶干法厌氧消化过程中理化参数以及产沼气特性的影响及产气最佳的C/N值厌氧消化过程中细菌群落结构多样性,研究采用牛粪与甘蔗叶以及啤酒厂滤泥这3种有机固废物混合并加入自行研究设计的发酵罐里进行厌氧消化,并采用Nested-PCR(巢式PCR)技术扩增消化过程中细菌的16S r DNA V3区域,并通过DGGE方法分析初始C/N值为15.02厌氧消化过程细菌群落的多样性。实验结果表明,初始C/N值为15.02的混合物料产甲烷最佳,最高累计产气量为1 748.63 m L·kg~(-1),甲烷最高含量为59.26%,最高日产甲烷量为13.58 m L·kg~(-1);通过研究最佳C/N值为15.02的细菌多样性,可知在厌氧消化过程中细菌数量和群落结构丰富多样,样品间的细菌群落结构差异性相对较大,样品间存在与产沼气相关的共有的优势细菌。
In order to study the effect of different initial C / N ratio on the physicochemical parameters and biogas production characteristics during the dry anaerobic digestion of sugarcane leaves and the bacterial community structure diversity during the anaerobic digestion of C / N with the best initial C / N value, The study used cow dung and sugar cane leaf and brewery mud three kinds of organic waste were mixed and added to study the design of the fermentation tank for anaerobic digestion and use of Nested-PCR (nested PCR) technology to amplify bacteria during digestion Of 16S rDNA V3 region, and the diversity of bacterial communities during anaerobic digestion with an initial C / N value of 15.02 was analyzed by the DGGE method. The experimental results showed that the best methane yield was obtained from the mixture with the initial C / N value of 15.02. The maximum cumulative gas production was 1 748.63 m L · kg -1, the maximum methane content was 59.26% and the maximum daily methane production was 13.58 m L · kg -1. By studying the diversity of bacteria with the best C / N value of 15.02, we can see that the bacterial population and community structure are rich and diverse during the anaerobic digestion, and the bacterial community structure is relatively different , There are common dominant bacteria associated with biogas production in the samples.