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作者用影像分析器对面神经各段的断面进行定量测量并加以分析。取豚鼠的颞骨立即置入2.5％戊二醛固定六小时,然后将面神经干和鼓索神经取出.再经1％OsO_41小时处理后脱水包埋,切片厚度约为0.5μm,经甲苯胺蓝染色,在500倍显微镜下将每张切片分成8～10部分拍照,然后放大数倍,用影像分析器测量有髓神经纤维的数目和直径。作者选择了迷路段、鼓室段、乳突段及鼓索神经的横断面切片。
The author uses image analyzer to quantitatively measure and analyze the sections of facial nerve. The temporal bone of guinea pigs were immediately fixed with 2.5% glutaraldehyde for 6 hours, then the facial nerve trunk and the chordoma were removed, then dehydrated and embedded in 1% OsO_41 hour, and the section thickness was about 0.5μm. Toluidine blue staining , Under the microscope at 500 times each slice is divided into 8 to 10 part of the picture, and then magnified several times, with an image analyzer to measure the number and diameter of myelinated fibers. The authors selected the labyrinthine segment, tympanic segment, mastoid segment, and cross section of the chorda.