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【方法】①低温过程:将马粪、稻草、干草和紫花苜蓿剁碎,干的过25毫米筛。切碎的原料预先浸透,并通过3级锤磨。但预先浸透的稻草捆和马粪会导致矿质养分和氮素大量流失,应尽量采用干料,再加入适量的水。标准生物活化剂占蘑菇商业堆料湿重的5～10％。所有的试验,仅采用脱水羽毛粉作氮素添加物,脱水羽毛粉含12.5％氮素。后发酵前的填料为28公斤/米~2,装在4英寸深的浅木盆里,不宜压得过紧,接种时将两木盆的料并到一个盆。在后发酵期,床温只比气温高1～2℃,不断通入新鲜空气和蒸气。除了短期(4小时)达60℃外,床温维持在52～55℃。接种前没有冷却阶段,将木盆直接从52～55℃的菇房移至室外棚下放2～3小时,接种量为盆里鲜料的1％(W/W),发菌温度
【Method】 ① low temperature process: horse manure, straw, hay and alfalfa minced, dried over 25 mm sieve. The shredded material is pre-soaked and passed through a 3-stage hammer mill. However, the pre-soaked straw bales and horse dung will cause a great loss of mineral nutrients and nitrogen. Drying materials should be used as far as possible and water should be added as appropriate. The standard biological activator accounts for 5 to 10% of the wet weight of mushroom commercial stacker. In all experiments, only dehydrated feather meal was used as the nitrogen addition and the dehydrated feather meal contained 12.5% nitrogen. After the fermentation before the filler is 28 kg / m ~ 2, installed in 4 inches deep shallow tub, should not be pressed too tight, the two tubs were inoculated when the material and to a basin. In the post-fermentation period, the temperature of the bed is only 1 ~ 2 ℃ higher than the temperature, and the fresh air and steam are continuously introduced. The bed temperature was maintained at 52-55 ° C except for short term (4 hours) up to 60 ° C. There was no cooling stage prior to inoculation. The tubs were moved directly from the mushroom house at 52-55 ° C to the outdoor shed for 2 to 3 hours. The inoculum size was 1% (W / W) fresh material in the pots,