育亨宾碱的正离子电喷雾离子阱质谱研究

• 作 者: 李鹏 陈琴华 陈富超
• 机 构: 湖北医药学院附属东风医院,
• 出 处: 中药材
• 发表日期: 2011年01期
• 关键词: 碎片离子 育亨宾碱 质谱研究 化合物 结构修饰 电喷雾 离子阱 裂解途径 正离子 质谱技术

目的:采用电喷雾离子阱(ESI-MS)质谱技术对育亨宾碱的结构和裂解途径进行研究。方法:采用ESI-MS获得了m/z 355[M+H]+,采用ESI-MS2获得了m/z 212和m/z 144等碎片离子。结果:ESI-MS2主要有两种裂解途径,主要在吡啶环裂解,并对m/z 212和m/z 144特征碎片离子进行ESI-MS3质谱研究,归属了其主要特征碎片离子,分析和讨论了该化合物的结构和质谱特征。结论:为进一步研究育亨宾碱化合物的体内代谢过程与结构修饰提供实验依据。

OBJECTIVE: To study the structure and lysis pathway of yohimbine by electrospray ion trap (ESI-MS) mass spectrometry. Methods: m / z 355 [M + H] + was obtained by ESI-MS, and fragment ions m / z 212 and m / z 144 were obtained by ESI-MS2. Results: ESI-MS2 has two major cleavage routes, mainly pyrolyzed in the pyridine ring, and ESI-MS3 mass spectrometry studies on the m / z 212 and m / z 144 characteristic fragment ions are assigned to their main characteristic fragment ions for analysis and discussion The structure and mass spectral characteristics of the compound. Conclusion: This study provides an experimental basis for further study on the metabolism and structural modification of yohimbe alkaloids in vivo.