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目的研究血清中有机氯农药水平及谷胱甘肽转移酶M1(GSTM1)基因多态性对女性乳腺癌患病风险的交互作用。方法自2006年9月至2007年10月,在唐山市5所二甲以上医院收集经病理学确诊的乳腺癌患者70例。选取病例所在医院同期住院的女性患者,年龄相差不超过2岁,按相近居住地区进行1∶1配比作为对照。采用气相色谱-电子捕获(GC-ECD)方法检测血清中有机氯农药[滴滴涕(DDT)包括4种同分异构体:p,p’-DDE、p,p’-DDT、o,p’-DDT、p,p’-DDD;六六六(HCH)包括4种同分异构体:α-HCH、β-HCH、γ-HCH、δ-HCH]残留水平,采用聚合酶链反应(PCR)检测GSTM1基因多态性,根据交互作用系数(γ)判断交互作用存在与否以及不同的基因-环境作用类型。结果GSTM1基因多态性与DDT及HCH间存在一定的交互作用,交互作用系数分别为1.237、1.379,交互作用分别表现为次相乘模型和超相乘模型。结论乳腺癌的发生是环境和遗传因素综合作用的结果。GSTM1基因多态性与环境危险因素DDT、HCH的暴露在乳腺癌发生中存在一定的交互作用。
Objective To study the interaction between serum levels of organochlorine pesticides and glutathione transferase M1 (GSTM1) gene polymorphism in the risk of breast cancer in women. Methods From September 2006 to October 2007, 70 patients with pathologically diagnosed breast cancer were collected from more than 5 hospitals in Tangshan. Select the same hospital where the case of female patients hospitalized, the difference in age does not exceed 2 years old, according to similar living area ratio of 1: 1 as a control. DETERMINATION OF ORGANOCHLORINE PESTICIDES IN SERUM BY GC-ECD [DDT] includes four isomers: p, p’-DDE, p, p’-DDT, -DDT, p, p’-DDD; the residues of HCH including four isomers: α-HCH, β-HCH, γ-HCH and δ-HCH] were determined by polymerase chain reaction PCR) to detect GSTM1 gene polymorphisms, and to determine the presence or absence of interaction and the different genotypes of environmental effects based on the interaction coefficient (γ). Results There was a certain interaction between GSTM1 gene polymorphism and DDT and HCH. The interaction coefficients were 1.237 and 1.379, respectively. The interactions were sub-multiplicative and hyper-multiplicative. Conclusion The occurrence of breast cancer is the result of a combination of environmental and genetic factors. GSTM1 gene polymorphism and environmental risk factors DDT, HCH exposure in the occurrence of breast cancer there is a certain interaction.