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目的 研究不同温度热诱导后 Ｈｅ Ｌａ 细胞热休克蛋白（ｈｅａｔ ｓｈｏｃｋ ｐｒｏｔｅｉｎ７０， Ｈ Ｓ Ｐ７０）蛋白动态表达。方法 Ｈｅ Ｌａ 细胞经过不同温热（４１℃ １ ｈ、４２℃ １ ｈ 、４３℃ １ ｈ、４５℃ ３０ ｍ ｉｎ）的诱导，以 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ及 Ｗｅｓｔｅｒｎ 印迹方法检测细胞裂解液中 Ｈ Ｓ Ｐ７０ 蛋白表达。结果 热诱导后 Ｈ Ｓ Ｐ７０ 蛋白表达的高峰出现时间随诱导温度的提高逐渐后移（如：４１℃ ８ ｈ、４３℃ １６ ｈ），且 ４１℃、４２℃热诱导后蛋白表达于诱导后 ８～１６ ｈ 期间有持续高水平表达，而４５℃ ３０ ｍ ｉｎ 热诱导细胞大部分于诱导后即时死亡，且仅有低水平的 Ｈ Ｓ Ｐ７０ 表达；高表达后 Ｈ Ｓ Ｐ７０ 一般于诱导后２４～３２ ｈ 始恢复至正常细胞表达水平。结论 温热诱导（４１℃ １ ｈ）短时间内即可产生高表达量 Ｈ Ｓ Ｐ７０，而较激烈热诱导（４３℃ １ ｈ、４５℃ ３０ ｍ ｉｎ）仅产生少量延迟表达的 Ｈ Ｓ Ｐ７０ 蛋白，且导致大量 Ｈｅ Ｌａ 细胞死亡，此为临床肿瘤温热治疗提供了理论依据。
Objective To investigate the dynamic expression of heat shock protein70 (HSP70) in He La cells after heat induction at different temperatures. Methods HeLa cells were induced by different warming (41°C for 1 h, 42°C for 1 h, 43°C for 1 h, and 45°C for 30 min). Cell lysates were detected by S D S P A G E and Western blotting methods. In the HPS P70 protein expression. Results The peak appearance time of HSP70 protein expression after heat induction gradually shifted with the increase of the induction temperature (eg, 41°C, 8 h, 43°C, 16 h), and protein expression was induced after heat induction at 41°C and 42°C. 8 High-level expression was sustained during ~16 h, and most of the cells were induced to die immediately after induction at 45 °C for 30 min in heat, and there was only low level expression of H S P70. After high-expression, H SP70 was generally 24 to 24 hours after induction. 32 h return to normal cell expression levels. Conclusion Hyperthermia (41°C for 1 h) can produce high expression of H S P70 in a short time, while intense heat induction (43°C for 1 h, 45°C for 30 min) produces only a small amount of delayed expression of H S P70 protein. , And lead to a large number of He La cells, which provides a theoretical basis for clinical tumor hyperthermia treatment.