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目的:研究水通道蛋白-1在牛角膜内皮细胞中的表达及在角膜内皮液体转运中的作用。方法:用免疫组织化学方法,检测水通道蛋白-1在培养的牛角膜内皮细胞中的表达,并用水通道蛋白阻滞剂对氯汞苯磺酸酯(pCMBS)处理培养的牛角膜内皮细胞,观察处理前后角膜内皮细胞的渗透性水通透率(Pf)改变。结果:培养的牛角膜内皮细胞中有水通道蛋白-1的表达,主要位于细胞膜上。汞剂处理前后的角膜内皮细胞Pf分别为(0.044±0.005)cm/s(n=15)和(0.017±0.003)cm/s(n=15),其差异有统计学意义(P<0.01)。结论:水通道蛋白-1在牛角膜内皮细胞液体转运中起着重要作用,其表达的改变会导致角膜水肿和功能改变。
AIM: To investigate the expression of aquaporin-1 in bovine corneal endothelial cells and its role in corneal endothelial fluid transport. Methods: The expression of aquaporin-1 in cultured bovine corneal endothelial cells was detected by immunohistochemical method. The cultured bovine corneal endothelial cells were treated with pCMBS with water channel blocker, Observed before and after treatment of corneal endothelial cell permeability of water permeability (Pf) changes. Results: Aquaporin-1 was expressed in cultured bovine corneal endothelial cells, mainly located on the cell membrane. The Pf of corneal endothelial cells before and after the amalgam treatment were (0.044 ± 0.005) cm / s (n = 15) and 0.017 ± 0.003 cm / s (n = 15) . CONCLUSION: Aquaporin-1 plays an important role in the fluid transport of bovine corneal endothelial cells, and the alteration of its expression leads to corneal edema and functional changes.