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目的探讨大豆异黄酮对未成年小鼠卵巢发育影响,为雌性生殖发育毒性研究提供实验依据。方法21 d ICR小鼠48只,按体重随机分为对照组,低、中、高剂量大豆异黄酮组(50、100、200㎎/㎏),每日灌胃1次,持续5周,干预结束后测定血清性激素水平,观察阴门开放时间,计算卵巢脏器系数及各级卵泡构成比。结果高剂量大豆异黄酮组小鼠卵巢系数[(0.436±0.120)mg/g]低于对照组[(0.987±0.420)mg/g](P<0.05);高剂量大豆异黄酮组小鼠雌二醇水平[(32.96±12.83)pg/m L]明显低于对照组[(53.76±16.44)pg/m L](P<0.05),而孕酮在各组间差异无统计学意义;与对照组小鼠阴门开放时间[(33.50±2.68)d]比较,中剂量大豆异黄酮组小鼠阴门开放时间[(31.14±1.21)d]提前,差异有统计学意义(P<0.05);大豆异黄酮组小鼠成熟卵泡比例随着剂量增加呈下降趋势,而闭锁卵泡比例呈上升趋势,与对照组比较,高剂量大豆异黄酮组小鼠成熟卵泡比例下降,闭锁卵泡比例上升,差异有统计学意义(P<0.05)。结论断乳至性成熟期持续暴露大豆异黄酮,可干扰未成年小鼠卵巢发育。
Objective To investigate the effects of soy isoflavones on the development of ovary in young mice and provide experimental basis for the study of female reproductive and developmental toxicity. Methods Forty-one ICR mice were randomly divided into control group, low, medium and high dose soy isoflavones group (50,100,200㎎ / ㎏) on day 21 after intragastric administration for 5 weeks. After the end of the determination of serum sex hormone levels, open door to observe the time to calculate the coefficient of ovarian organs and the constituent ratio of follicles at all levels. Results Compared with the control group [(0.987 ± 0.420) mg / g] [(0.436 ± 0.120) mg / g], the ovarial coefficient of high dose soy isoflavone group mice was significantly lower than that of the control group The level of diol [(32.96 ± 12.83) pg / m L] was significantly lower than that of the control group (53.76 ± 16.44 pg / m L) (P <0.05), while there was no significant difference in progesterone between groups Compared with the control group, the opening time of the vulva in the mice [(33.50 ± 2.68) d] was earlier than that of the middle-dose soy isoflavones group [(31.14 ± 1.21) d], the difference was statistically significant (P <0.05) Compared with the control group, the proportion of mature follicles in isoflavone-treated mice decreased with the increase of dose and the proportion of atresia follicles was increased. Compared with the control group, the proportion of mature follicles in high-dose isoflavone-treated mice decreased and the proportion of atresia follicles increased Significance (P <0.05). CONCLUSION Continuous exposure of soy isoflavones during weaning to sexual maturation can interfere with the development of ovary in young mice.