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目的探讨2型糖尿病与载脂蛋白J(ApoJ)外显子7基因多态性的关系。方法用聚合酶链式反应-变性梯度凝胶电泳技术(PCR-DGGE)对60例2型糖尿病患者、60例健康对照者的ApoJ基因外显子7进行基因筛查。结果发现ApoJ基因外显子7在60例2型糖尿病病例组中46例为突变杂合型(76.67%),60例对照组中22例为突变杂合型(36.67%),ApoJ外显子7基因多态性在2型糖尿病病例组和健康对照组间存在显著性差异(P<0.01)。结论ApoJ外显子7基因多态性可能是2型糖尿病的遗传标记之一,可能与2糖尿病发病有关联。
Objective To investigate the relationship between type 2 diabetes and apolipoprotein J (ApoJ) exon 7 polymorphism. Methods ApoJ gene exon 7 of 60 type 2 diabetic patients and 60 healthy controls were genotyped by PCR-DGGE. The results showed that 46 cases of ApoJ gene exon 7 were mutation heterozygous (76.67%) in 60 cases of type 2 diabetes mellitus, 22 cases of mutation heterozygous (36.67%) in 60 cases of control group, ApoJ exon 7 gene polymorphism in type 2 diabetes patients and healthy controls there was a significant difference (P <0.01). Conclusion ApoJ exon 7 polymorphism may be one of the genetic markers of type 2 diabetes mellitus, which may be associated with the pathogenesis of type 2 diabetes.