论文部分内容阅读
目的观察n-3多不饱和脂肪酸(n-3PUFAs)对大鼠结直肠癌的影响。方法将60只SD结直肠癌大鼠随机分成对照组和实验组。实验组用n-3PUFAs进行灌胃,对照组用等量的0.9%氯化钠溶液灌胃。两组均用N-甲基-N-亚硝基脲(MNU)进行灌肠。观察大鼠的一般情况。16周后解剖大鼠,观察大鼠肿瘤的情况。HE切片观察肿瘤的病理,气相色谱方法测定两组大鼠红细胞膜n-3PUFAs的浓度。结果实验组大鼠的一般情况改变轻于对照组(P<0.01或0.05),红细胞n-3PUFAs浓度高于对照组(P<0.01),肿瘤发生率低于对照组(P<0.05),差异均有统计学意义。结论 n-3PUFAs能够改善大鼠的一般情况,预防SD大鼠结直肠癌的发生。
Objective To investigate the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs) on colorectal cancer in rats. Methods Sixty SD rats with colorectal cancer were randomly divided into control group and experimental group. The experimental group with n-3PUFAs gavage, the control group with the same amount of 0.9% sodium chloride solution gavage. Both groups were enema with N-methyl-N-nitrosourea (MNU). Observe the general situation of rats. After 16 weeks, the rats were dissected to observe the tumor in rats. HE sections were used to observe the pathology of the tumor. Gas chromatographic method was used to determine the concentration of n-3PUFAs in the erythrocyte membrane of the two groups. Results The general changes of rats in the experimental group were less than those in the control group (P <0.01 or 0.05), the concentrations of erythrocyte n-3 PUFAs in the control group were significantly higher than those in the control group (P <0.01) All have statistical significance. Conclusion n-3PUFAs can improve the general condition of rats and prevent the occurrence of colorectal cancer in SD rats.