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目的观察变异链球菌密度感应信号系统相关基因ComD基因缺失前后变形链球菌生物膜成熟初期的变化情况,探讨ComD基因对变异链球菌形成菌斑生物膜的影响,从而为防治龋病提供实验依据和新方法。方法分别构建标准菌和缺陷菌在离体模型上的培养模型,通过扫描电镜观察两个实验组在6 h、12 h、24 h三个不同时间点的生物膜形成情况。结果在三个不同时间点观察生物膜形成,扫描电镜结果显示培养6 h和12 h两种菌株形成生物膜无明显差异,培养24 h后发现ComD缺陷菌形成的生物膜中细菌定植较少且生物膜孔隙大而疏松。结论与标准菌株相比,ComD基因缺陷的变异链球菌在成熟初期生物膜表型有明显缺陷,因此ComD基因可以作为防治龋病的靶点之一。
Objective To observe the changes of S. mutans biomembrane before and after the deletion of ComD gene of Streptococcus mutans density-sensitive signaling system, and to explore the effect of ComD gene on the formation of plaque biofilm in Streptococcus mutans so as to provide experimental evidence for prevention and treatment of dental caries new method. Methods The culture model of the standard bacteria and the defective bacteria on the in vitro model was constructed. The biofilm formation of the two experimental groups at different time points of 6 h, 12 h and 24 h was observed by scanning electron microscopy. Results Biofilm formation was observed at three different time points. Scanning electron microscopy showed no significant difference in biofilm formation between the two strains cultured for 6 h and 12 h. After culturing for 24 h, bacterial colonization by ComD-deficient bacteria was found to be rare Biofilm pores large and loose. Conclusion Compared with the standard strains, ComD gene-deficient Streptococcus mutans have obvious defects in the early stage of biofilm development. Therefore, ComD gene can be used as one of the target of prevention and treatment of dental caries.