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目的 研究贫困山区孕期巨细胞病毒 (cytomegalovirus,CMV)感染流行病学状况 ,分析肿瘤坏死因子α (tumornecrosisfactorα ,TNFα)与CMV宫内感染的关系。方法 用ELISA和PCR方法检测陕西贫困山区30 5例正常孕妇外周血特异性抗CMVIgM抗体及CMVDNA、 48例孕期CMV感染者及 6 0例孕期未感染CMV者产后 2周内乳汁及其所分娩新生儿尿液CMVDNA ;并采用双抗体夹心ELISA法检测孕妇及乳母外周血中TNFα的水平。结果 陕西贫困山区孕期CMV活动性感染率 19 5 9% ,孕期CMV活动性感染及有宫内传播、乳汁排泄CMV者外周血TNFα水平显著高于未感染者 (P <0 0 5 )。结论 贫困山区孕期CMV感染率较国内外报道率高 ;TNFα在孕期活动性CMV感染及宫内传播、产后乳汁排泄CMV中可能起着重要作用。
Objective To study the prevalence of cytomegalovirus (CMV) infection in poor mountainous areas and to analyze the relationship between tumor necrosis factor α (TNFα) and intrauterine infection of CMV. Methods Peripheral blood specific anti-CMV IgG antibody and CMVDNA were detected in 30 5 normal pregnant women in poverty-stricken mountainous areas of Shaanxi Province by ELISA and PCR. 48 pregnant women with CMV infection and 60 pregnant women with non-infected CMV during pregnancy were tested for lactation and neonatal delivery The urine of patients with CMVDNA; and double antibody sandwich ELISA method to detect the level of TNFα in the peripheral blood of pregnant women and nursing mothers. Results In rural poverty-stricken areas of Shaanxi Province, the infection rate of CMV during pregnancy was 19 59%. CMV activity during pregnancy and intrauterine transmission of CMV were significantly higher than those of non-infected patients (P <0.05). Conclusion The infection rate of CMV during pregnancy in impoverished mountainous area is higher than that reported in China and abroad. TNFα may play an important role in active CMV infection and intrauterine transmission during pregnancy, and postpartum CMV excretion.