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迄今,有关个体的疼痛程度和炎症程度之间的精确关系一直存在争论,主要原因是缺乏能够同时反映多种痛(尤其是可鉴别疼痛即早期和持续期)的炎症模型以及定量方法的合理应用。因此,本研究在啮齿类动物评价了外周皮下组织致炎后炎症水肿与伤害性反应以及痛敏之间的相关性。为了更好地认识炎症特异性特征在治疗中的价值,我们对非甾体类抗炎药的作用效果也进行了评价。将一个剂量的蜜蜂毒(0.05mg/0.025ml)注入12个近交系(129P3/J、A/J、AKR/J、BALB/cJ、C3H/HeJ、C57BL/6J、C57BL/10J、C58/J、CBA/J、DBA/2J、RIIIS/J和SM/J)小鼠或6个剂量的蜜蜂毒(0.001、0.005、0.01、0.05、0.1、0.2mg/0.05ml)注入远交系(Sprague-Dawley)大鼠的一侧足底皮下,分别检测自发伤害性反应、热和机械性痛敏,以及炎症的水肿和局部皮温,然后对组间和组内数据进行相关性分析。此外,观察非甾体类抗炎药吲哚美辛对痛和炎症的作用效果。结果显示:(1)炎症水肿程度与注射侧自发缩足反射次数、舔足抬足时间等伤害性反射程度呈高度正相关(P≤0.003),而与热或机械性痛敏的程度没有相关性;(2)吲哚美辛(0.5、2.5、25mg/kg,i.p.,稀释于60%二甲基桠枫)可以剂量依赖性地抑制炎症水肿和自发伤害性反应,但是对热或机械性痛敏却只有在最高剂量下才有作用。这些结果提示,炎症水肿过程可能只参与动物受炎症刺激而引起的即早自发伤害性反应过程,而不参与与临床更加密切相关的痛敏过程。这个分析结果为确定抗炎治疗有益于缓解多种炎性痛中的哪个靶表证提供了一个有用的分析方法。
To date, there has been debate about the precise relationship between the degree of pain and the degree of inflammation in an individual, mainly due to a lack of an appropriate model of inflammation that can reflect multiple pains, especially early and prolonged pain, as well as the rational use of quantitative methods . Therefore, the present study evaluated the correlation between inflammatory edema and nociceptive response and nociception in rodents after inflammation of peripheral subcutaneous tissue. To better understand the value of inflammatory-specific traits in treatment, we also evaluated the efficacy of NSAIDs. A dose of bee venom (0.05 mg / 0.025 ml) was injected into 12 inbred lines (129P3 / J, A / J, AKR / J, BALB / cJ, C3H / HeJ, C57BL / 6J, C57BL / J, CBA / J, DBA / 2J, RIIIS / J and SM / J mice or 6 doses of bee venom (0.001,0.005,0.01,0.05,0.1,0.2 mg / -Dawley rats were subcutaneously subcutaneously screened for spontaneous nociception, thermal and mechanical hyperalgesia, and inflammatory edema and local skin temperature, respectively, and correlation analysis between groups and groups was performed. In addition, observe the effect of non-steroidal anti-inflammatory drugs indomethacin on pain and inflammation. The results showed that: (1) The degree of inflammatory edema was positively correlated with the degree of reflex reflex (P≤0.003), such as the number of reflex reflex at the injection side and lifted foot licking time, but not with the degree of thermal or mechanical hyperalgesia (2) Indomethacin (0.5, 2.5, 25 mg / kg, ip, diluted in 60% dimethyl squar) inhibited the inflammatory edema and spontaneous nociception in a dose- Pain sensitivity but only in the highest dose has a role. These results suggest that the process of inflammatory edema may be involved only in the process of early spontaneous nociception induced by inflammatory stimuli and not involved in the more pain-sensitive process associated with clinical. The results of this analysis provide a useful method of analysis for determining which target anti-inflammatory treatment is beneficial in alleviating a variety of inflammatory pain.