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以成熟稗草种子作为外植体,通过去除大部分胚乳和消毒处理,置于诱导培养基(MS+0.6 g·L~(-1)水解酪蛋白+2.0 mg·L~(-1)2,4-D+0.2 mg·L~(-1) 6-BA+0.2 mg·L~(-1) NAA)上,黑暗条件下培养7 d后超过80%种子长出愈伤组织。将愈伤组织置于继代培养基(MS+0.2 mol·L~(-1)甘露醇+0.5 mg·L~(-1) 2,4-D+0.5 mg·L~(-1) 6-BA)上,光照条件下培养7 d后有超过80%的愈伤组织成活。将继代后的愈伤组织转入分化培养基(MS+0.6 g·L~(-1)水解酪蛋白+0.2 mg·L~(-1) ZT+2.0 mg·L~(-1) NAA)上,培养14 d后超过60%的愈伤组织分化成苗。将分化苗转入生根培养基(1/2MS+0.6 g·L~(-1)水解酪蛋白)上,7 d后有超过90%的分化苗生根。试管苗经3 d炼苗后移栽入土壤,成活率达100%。
The mature barnyardgrass seeds were used as explants, and most of the endosperm was removed and disinfected, and placed in induction medium (MS + 0.6 g · L -1 hydrolyzed casein + 2.0 mg · L -1) , 4-D + 0.2 mg · L -1 (-1) 6-BA + 0.2 mg · L -1 NAA). More than 80% of the seeds grew callus after 7 days of darkness cultivation. The callus was placed in subculture medium (MS + 0.2 mol·L -1 mannitol + 0.5 mg · L -1 2,4-D + 0.5 mg · L -1) 6 -BA), more than 80% callus survived after 7 days of light cultivation. The callus after subculture was transferred into differentiation medium (MS + 0.6 g · L -1 hydrolyzed casein + 0.2 mg · L -1 ZT + 2.0 mg · L -1 NAA ), More than 60% callus differentiated into seedlings after 14 days of culture. Differentiated plants were transferred to rooting medium (1 / 2MS + 0.6 g · L -1 hydrolyzed casein), and more than 90% of the differentiated shoots were rooted after 7 days. Test-tube seedlings by 3 d after transplanting into the soil hardening, survival rate of 100%.