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目的 探讨采用蜕膜建立人子宫内膜异位症移植动物模型的可行性。方法 将早孕期蜕膜组织种植于NOD- SCID鼠腹部皮下,随机分成4组,分别在2、4、6、8周,取出其皮下移植物送病理检查,并用免疫组织化学的方法观察其形态学、增殖活性方面有无改变。结果 16只小鼠均存活,有15只小鼠皮下病灶经HE染色可见明显的子宫内膜腺体和间质,成功率为93. 75%。腺上皮细胞角蛋白、ki67染色均阳性,基质细胞波形蛋白染色阳性, 8周移植物中一些基质细胞减少区域的腺上皮细胞波形蛋白染色阳性。结论 采用人蜕膜建立NOD- SCID小鼠子宫内膜异位症模型成功率高,模型形状显著且稳定。
Objective To explore the feasibility of using decidua to establish an animal model of human endometriosis transplantation. Methods Decidua in early pregnancy were implanted subcutaneously into the abdomen of NOD-SCID mice and randomly divided into 4 groups. The subcutaneous implants were removed at 2, 4, 6, and 8 weeks respectively for pathological examination. The morphological changes were observed by immunohistochemistry Learn, proliferative activity has changed. Results 16 mice survived, 15 mice subcutaneous lesions by HE staining obvious endometrial glands and stroma, the success rate was 93.75%. Glandular epithelial keratin, ki67 staining were positive, stromal vimentin positive staining, glandular epithelial vimentin staining in some of the stromal cells in the 8-week graft-positive area. Conclusion The model of endometriosis in NOD-SCID mice established by human decidua has a high success rate and the model shape is remarkable and stable.