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多通道流动电泳是一项新型制备电泳技术。电泳过程在5腔室电泳槽中进行,各腔室之间用膜隔开,中间腔室为进样室,其两侧依次为冲洗室和电极室。当蛋白质混合物被连续引入进样室时,带电组分在电场作用下迁移过膜进入其对应的冲洗室,被载流冲出,中性组分则被进样流从进样室出口带出,从而实现分离。多通道流动电泳技术适宜于分离等电点有较大差异的混合物,可广泛应用于各类生物产品如蛋白质、酶、抗体及其它生物技术产品的大规模分离纯化过程。
Multichannel flow electrophoresis is a new type of preparative electrophoresis technique. The electrophoresis process was carried out in a 5-chamber electrophoresis tank with membranes separated by a membrane. The middle chamber was a sample injection chamber, and the two sides were followed by a wash chamber and an electrode chamber. When the protein mixture is continuously introduced into the sample chamber, the charged components migrate through the membrane under the action of an electric field into their corresponding wash chambers, are carried by the carrier stream, and the neutral component is carried by the sample stream from the sample chamber outlet , In order to achieve separation. Multi-channel flow electrophoresis technology is suitable for the separation of isoelectric point have a greater difference between the mixture can be widely used in various biological products such as proteins, enzymes, antibodies and other biotechnology products, large-scale separation and purification process.