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用免疫组织化学方法结合图像分析研究Ｃ５７ＢＬ／６Ｊ小鼠小脑皮质和脊髓内突触素（ｐ３８）的年龄变化以及长期适量运动（跑转笼）对突触素年龄变化的影响。以同年龄对照组心重／体重比率均值的２信标准差作为运动有效标准。结果显示，２４月龄鼠小脑皮质Ⅰ、Ⅴ叶分子层突触素免疫反应产物光密度值（ＣＯＤ值）明显小于１３月龄鼠（Ｐ＜０．０１），后者ＣＯＤ值小于５月龄鼠（Ｐ＜０．０１）；Ｌ４～Ｓ１段脊髓灰质Ⅱ、Ⅴ、Ⅶ层，突触素年龄相关的变化不明显（Ｐ＞０．０５）。５月龄小鼠经过８和１９个月运动后，小脑分子层突触素免疫反应产物均较对照组显著增高（Ｐ＜０；０１），脊髓灰质Ⅱ、Ⅴ层只在１９个月运动组显著增高（Ｐ＜０．０５，Ｐ＜０．０１）。表明从青年开始的长期适量运动能够防止随年龄增加的小脑皮质突触素的丢失，促进小脑皮质和脊髓灰质内突触素的合成。
Immunohistochemistry and image analysis were used to investigate the age-related changes of synaptophysin (p38) in the C57BL / 6J mice and the effect of long-term moderate exercise on the changes of synaptophysin age. The standard of 2-letter standard deviation of the mean of body weight / body weight ratio of the same age control group was taken as the effective standard of exercise. The results showed that the optical density (COD) of the synaptophysin immunoreactive products in the I and V leaf layers of 24-month-old mice was significantly lower than that of 13-month-old mice (P <0.01) (P <0.01). There was no significant difference in age-related changes of synaptophysin between L4 and S1 spinal cord layers Ⅱ, Ⅴ and Ⅶ (P> 0.05). After 8 months and 19 months of exercise, the synaptophysin immunoreactive products in cerebellar molecular layer of 5-month-old mice were significantly higher than those of the control group (P <0.01). The gray matter Ⅱ and Ⅴ layers of spinal cord were only in the 19-month exercise group Significantly increased (P <0.05, P <0.01). Suggests that long-term moderate exercise starting from youth can prevent the loss of synaptophysin in the cerebellar cortex with increasing age, and promote the synthesis of synaptophysin in the cerebellar cortex and the spinal gray matter.