Stereological method for objectively quantifying myelin sheaths in the rat hippocampus

• 作 者: Lei Zhang Wei Lu Shu Yang Lin
• 机 构: DepartmentofHistologyandEmbryology,LaboratoryofStemCellandTissueEngineering
• 出 处: 中国神经再生研究：英文版
• 发表日期: 2011年31期
• 关键词: 量化考核 体视学 髓鞘 海马 大鼠 透射电子显微镜 随机抽样 正交测量 myelin sheaths diameter perimeter thickness
• 基金项目: the National Natural Science Foundation of China, No. 30973155.Acknowledgments： We thank all the staff in the Department of Electron Microscopy of the Third Military Medical University, China, for tec

In the present study,tissue blocks were randomly sampled from the entire hippocampus of 6-week-old Long-Evans rats.Isotropic,uniform and random sections,60 nm thick,were prepared by isector.Fifteen fields of view were randomly selected for each section an