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在离子液体([EMIM]Br)中,将聚邻苯二胺(PoPD)-多壁碳纳米管(MWCNTs)复合物原位电聚合到金电极(Au)表面,利用纳米金固载髓过氧化物酶(MPO)抗体,构建了一种用于MPO检测的无需标记的电流型免疫传感器.采用循环伏安法(CV)和扫描电子显微镜(SEM)对修饰过程进行表征.探讨了邻苯二胺单体浓度、pH、孵育时间和孵育温度对该免疫传感器性能的影响.实验结果表明,该传感器在最适条件下对MPO响应良好,其线性范围为0.25～350 ng/mL,线性相关系数为0.9985,检出限为0.07 ng/mL.该电流型免疫传感器具有稳定性好、灵敏度较高、特异性好、结果准确可靠和可再生等优点,可应用于临床检测.
Poly (o-phenylenediamine) (MWCNTs) poly (o-phenylenediamine) (MWCNTs) complexes were electropolymerized on gold electrode (Au) surface in ionic liquids ([EMIM] Br) (MPO) antibody to construct a marker-free current-mode immunosensor for MPO detection.Characteristics of the modification were characterized by cyclic voltammetry (CV) and scanning electron microscopy (SEM) The concentration of diamine, pH, incubation time and incubation temperature on the performance of the immunosensor.The experimental results show that the sensor responds well to MPO under the optimal conditions with a linear range of 0.25-350 ng / mL and a linear correlation The coefficient is 0.9985, and the detection limit is 0.07 ng / mL.The current type immunosensor has the advantages of good stability, high sensitivity, good specificity, accurate and reliable results and reproducible results, which can be applied to clinical testing.