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目的:通过3种不同的动物体内血栓形成模型,考察罗勒不同提取物对血栓形成的影响,比较不同极性活性组分作用差别,进而探讨其有效作用成分类型及筛选最佳提取工艺。方法:采用小鼠肺血栓模型、FeC l3诱导大鼠颈动脉血栓模型及大鼠下腔静脉血栓模型3种实验性血栓模型,考察罗勒不同提取物的抗血栓形成作用。结果:罗勒不同提取物均可提高胶原蛋白-肾上腺素混合诱导剂所致肺血栓小鼠的存活率,延长FeC l3诱导大鼠颈动脉血栓形成时间,并减轻血栓湿质量,减轻结扎法所致下腔静脉血栓湿质量,且其中以乙醇为溶剂采用超声波提取法的罗勒提取物效果最为显著。结论:罗勒提取物有显著的抗血栓作用,其主要有效组分可能为某些极性较小的脂溶性黄酮类成分。
OBJECTIVE: To investigate the effect of different extracts of basil on thrombus formation in thrombosis models of three different animals, and to compare the differences in action of different polar active ingredients, and to explore the types of active ingredients and the optimal screening process. Methods: Three experimental thrombus models of pulmonary thrombus in mice, FeCl 3 -induced rat carotid artery thrombosis model and rat inferior vena cava thrombus model were used to investigate the antithrombotic effects of different extracts of basil. Results: Different extracts of basil could improve the survival rate of pulmonary thrombus induced by collagen-adrenergic inducer, prolong the time of FeCl 3 -induced carotid artery thrombosis, reduce the thrombus wet mass and relieve the ligation Inferior vena cava thrombosis wet quality, and ethanol as the solvent using ultrasonic extraction basil extract the most significant effect. Conclusion: Basil extract has a significant antithrombotic effect, and its main active component may be some fat-soluble flavonoids.