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冈比亚按蚊(Anopheles gambiae)是非洲重要的疟疾媒介,但至今缺乏经典的遗传标记物,使研究和分析自然发生的疟疾抗拒性基因非常困难,用诱变实验来寻找这种位点尚无报道。限制性酶切片段长度多态性(RFLP)是分析种间连锁关系的重要依据,某些遗传标记即在其中。根据染色体原位杂交绘出的基因图,完整地反映了细胞遗传学材料。由于二酚氧化酶基因A2(Dox)的突变,可设想由合子—卵囊包裹产生疟疾抗拒性表型,由2个冈比亚按蚊近交系间的回交,可以确定A2
Anopheles gambiae, an important malaria vector in Africa, has so far lacked classical genetic markers that make it very difficult to study and analyze naturally occurring malaria resistance genes. There have been no reports of such sites using mutagenesis experiments . Restriction endonuclease fragment length polymorphism (RFLP) is an important basis for the analysis of interspecies linkage, which is where some of the genetic markers. According to the chromosome mapping in situ hybridization map, a complete reflection of cytogenetic material. Due to mutations in the diphenoloxidase gene A2 (Dox), it is conceivable that the malaria resistant phenotype was encapsulated by the zygotic-oocyst, and the backcross between the two Anopheles gambiae angels can determine A2