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目的建立黄柏饮片的光谱成像指纹图谱,为其质量控制提供新的方法。方法应用电可控液晶滤光光谱成像装置,测定10种不同市售来源的黄柏饮片,光谱分辨率为5nm,光谱覆盖范围为480～680nm,空间分辨率为4000×4000。从成像光谱立方体中提取特征光谱,构建其指纹图谱;采用标准欧氏距离等聚类分析方法解析其指纹图谱。结果黄柏饮片的光谱成像指纹图谱用于其品种和质量的鉴定,结果与性状,显微及理化鉴定结果相吻合。结论光谱成像分析技术可用于中药指纹图谱的构建和质量评价,操作方法简便、快速、无损。
Objective To establish a spectral fingerprint of Phellodendron chinense Pieces and provide a new method for its quality control. Methods Electronically controllable liquid crystal (LC) spectral filters were used to determine the cortex plicata in 10 different commercial sources. The spectral resolution was 5 nm, the spectral coverage was 480-680 nm and the spatial resolution was 4000 × 4000. The characteristic spectrum was extracted from the imaging spectral cube and its fingerprint was constructed. The fingerprint of the fingerprint was analyzed by clustering analysis such as standard Euclidean distance. Results The spectral imaging fingerprints of Phellodendron chinense were used for the identification of their varieties and qualities. The results were in good agreement with the traits, microscopic and physical and chemical identification results. Conclusion The technique of spectral image analysis can be used for the construction and quality evaluation of traditional Chinese medicine fingerprinting. The operation method is simple, rapid and non-destructive.