论文部分内容阅读
在对天然产物的抗癌或细胞毒作用和(或)它们的细胞生长抑制活性的初筛试验中,常使用诸如大鼠白细胞L-1210,L5178Y,P388,人癌KB细胞和大鼠黑色素瘤细胞B16等体外培养的肿瘤细胞进行试验。本试验观察了46种生药(230个提取物试样),51种巴拉圭的生药(53种提取物试样)和273种植物(322种提取物试样)对人癌KB细胞系的作用及其抑制率。对那些具有较强抑制活性的提取物则计算出IC_(50),试验用药的提取:先用甲醇提三次,残渣或甲醇溶解部分用
In primary screening tests on the anticancer or cytotoxic effects of natural products and / or their cytostatic activity, various cytokines such as rat leukocytes L-1210, L5178Y, P388, human cancer KB cells and rat melanoma Cell B16 and other in vitro cultured tumor cells were tested. In this experiment, the effect of 46 crude drugs (230 extract samples), 51 Paraguayan crude drugs (53 extract samples) and 273 species (322 extract samples) on the human cancer KB cell line was observed. Its inhibition rate. For those with strong inhibitory activity of the extract was calculated IC 50 (50), the experimental drug extraction: first with methanol to mention three times, the residue or methanol to dissolve part with