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目的:观察电针配合贯叶连翘提取物对抑郁大鼠行为学和脑微循环的影响,明确针药结合疗法的依据和可能机制。方法:SD雌性大鼠随机分为正常组、模型组、低剂量组、低剂量+电针组、高剂量组、高剂量+电针组,每组10只。采用慢性应激刺激法复制抑郁大鼠模型。贯叶连翘灌胃给药,其低、高剂量分别为10 mg/kg和20 mg/kg。电针“百会”“哑门”20 min,每天1次,连续治疗14 d。最后通过糖水消耗、开场活动、Morris水迷宫实验进行行为学评价,同时利用激光多普勒观察鼠额叶微循环。结果:与正常组比较,模型组大鼠可见糖水偏好程度显著降低,开场活动中也见水平和垂直活动显著减少;Morris水迷宫实验中模型组大鼠运动轨迹总路程、潜伏期、远站台区的轨迹路程和运动时间显著增加,平均速度、近/远站台区路程比、近/远站台区时间比显著减少;脑微循环血流量显著减少(均P<0.01)。与模型组比较,低剂量组、低剂量+电针组、高剂量组和高剂量+电针组均能改善抑郁大鼠的糖水消耗、开场活动以及Morris水迷宫实验中的运动轨迹总路程、潜伏期、远站台时间、运动速度和近/远站台区时间比例(P<0.05,P<0.01)。糖水消耗、开场水平活动和Morris水迷宫实验中的轨迹总路程、潜伏期、远站台区轨迹路程、远站台区时间等指标,高剂量+电针组与低剂量组比较,差异有统计学意义(P<0.05)。而高剂量组和低剂量组比较,差异无统计学意义(P>0.05)。低剂量+电针组和高剂量+电针组均可见脑微循环血流量的显著改善(P<0.01),而低、高剂量组均未见改善(P>0.05)。结论:给药组和给药配伍电针组均对抑郁大鼠有较好的治疗作用,但单纯的药物治疗并不能改善脑微循环血流量,而在此基础上配合电针治疗,则可见脑微循环血流量的显著改善。
Objective: To observe the effects of electroacupuncture combined with Hypericum perforatum on behavioral and cerebral microcirculation in rats with depression, and clarify the basis and possible mechanism of acupuncture combined with drug therapy. Methods: SD female rats were randomly divided into normal group, model group, low dose group, low dose + EA group, high dose group and high dose + EA group, with 10 rats in each group. Chronic stress stimulation was used to replicate the depressive rat model. Hypericum perforatum intragastric administration, the low and high doses were 10 mg / kg and 20 mg / kg. Electric acupuncture “Baihui ” “dumb ” 20 min, 1 day, continuous treatment 14 d. Finally, through behavioral evaluation of sugar consumption, opening activity and Morris water maze test, the mouse frontal microcirculation was observed by laser Doppler. Results: Compared with the normal group, the degree of preference of sugar in the model group was significantly decreased, and the horizontal and vertical activities were also significantly decreased in the opening activity. In the Morris water maze test, the total distance traveled, latency, far station area The path distance and exercise time increased significantly, while the average speed, the ratio of distance to near / far stations and the time ratio of near / far stations were significantly decreased. The cerebral microcirculation blood flow decreased significantly (both P <0.01). Compared with model group, low dose group, low dose + electroacupuncture group, high dose group and high dose + electroacupuncture group could improve the total sugar and water consumption, opening activity and the total distance of movement track in Morris water maze test in depressed rats, (P <0.05, P <0.01), latency, distance from station to station, speed of movement and time in near / far stations. Water consumption, opening level activity, Morris water maze test, the total distance of the trail, the incubation period, the distance traveled in the far station area and the time of the remote station area, the difference between the high dose + EA group and the low dose group was statistically significant ( P <0.05). However, there was no significant difference between high-dose group and low-dose group (P> 0.05). Both low-dose + electro-acupuncture and high-dose + electro-acupuncture showed a significant improvement of cerebral microcirculation blood flow (P <0.01), but no improvement was observed in both low and high dose groups (P> 0.05). Conclusion: Both the administration group and the electro-acupuncture combined with electro-acupuncture have better therapeutic effect on depression in rats, but the simple drug treatment can not improve cerebral microcirculation blood flow, and on this basis with the electro-acupuncture treatment, it can be seen Significant improvement in cerebral microcirculation blood flow.