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目的对辽宁省丹东市2015年8月发生的2例间日疟疫情调查处置进行描述和分析,指导继续开展消除疟疾工作。方法收集病例基本信息、流行病学资料、疫点自然情况、媒介监测情况等;收集本次疫情及既往间日疟病例全血,针对间日疟裂殖子表面蛋白基因进行套式PCR扩增并对产物测序分析。结果 2例病例为间日疟病例,主动病例搜索快速检测试验(RDT)检测病例周围居民467人,PCR检测103人,结果均为阴性;表面蛋白基因测序分析显示本次2例病例病原体间日疟裂殖子核酸序列高度同源,而与本省前几年本地病例则同源性不高,感染来源难以确定。结论 2例病例为本地感染病例,丹东地区存在传疟媒介,下一步应加大监测力度,继续调查感染来源;同时密切关注输入性病例,防止二代传播。
Objective To describe and analyze the investigation and investigation of Vitex malaria in two cases in August 2015 in Dandong City, Liaoning Province, and to guide the continuation of malaria elimination work. Methods The basic information of cases, epidemiological data, the natural conditions of epidemic sites and the media monitoring were collected. The whole blood samples of this epidemic and the past were collected and nested PCR was performed on the gene of Plasmodium vivax The product was sequenced and analyzed. Results Two cases were Plasmodium vivax, 467 residents were detected by RDT, and 103 were detected by PCR. The results of surface protein gene sequencing showed that there was no significant difference between the two pathogens The malaria merozoite nucleic acid sequence is highly homologous, but the homology with the local cases in the province a few years ago is not high, the source of infection is difficult to determine. Conclusions Two cases were local cases of infection. There was malaria transmission media in Dandong. The next step should be to monitor the intensity of the infection and continue to investigate the source of infection. At the same time pay close attention to imported cases to prevent the spread of the second generation.