论文部分内容阅读
各种不同浓度的氟化钠早已广泛地被用于预防龋齿。使用细胞遗传学检测系统,曾检出过氟化钠具有诱发染色体的断裂作用,但其遗传学毒性的发现始终不一。本实验使用培养的人类外周血淋巴细胞,研究氟化钠对染色体、程序外DNA合成(UDS)和姊妹染色单体互换(SCES)的影响。在诱发染色体断裂作用的试验中,使用各种不同浓度氟化钠处理培养细胞,连续24小时,对每一浓度组,观察的细胞数达10,000个以上。
Various concentrations of sodium fluoride have long been widely used to prevent dental caries. Using cytogenetic detection system, sodium fluoride has been found to induce chromosome rupture, but the discovery of genetic toxicity has always been different. This experiment used cultured human peripheral blood lymphocytes to study the effects of sodium fluoride on chromosomal, extra-procedural DNA synthesis (UDS) and sister chromatid exchange (SCES). In experiments that induced chromosome rupture, cultured cells were treated with various concentrations of sodium fluoride for 24 hours consecutively and the number of cells observed was 10,000 or more for each concentration group.