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[目的]了解氟铃脲的遗传毒性作用。[方法]选取55只小鼠,随机分成实验组、阴性对照组和阳性对照组。实验组以不同LD50剂量氟铃脲对小鼠经口灌胃染毒,阴性对照组以5g/L羧甲基纤维素钠溶液经口灌胃,阳性对照组以40mg/kg剂量磷酰胺腹腔注射。末次染毒6h后颈椎脱臼处死动物,按常规制片制备骨髓细胞和睾丸初级精母细胞染色体,分析小鼠骨髓细胞和睾丸初级精母细胞染色体畸变率。[结果]氟铃脲各剂量染毒组雄性小鼠骨髓细胞和初级精母细胞染色体畸变率与阴性对照组比较差异均无统计学意义(χ2=2.285,0.502,0.144,0.198,0.159,0.471,1.003,P﹥005),各剂量染毒组初级精母细胞常染色体及性染色体早熟分离发生率与阴性对照组比较差异也均无统计学意义(χ2=1.672,0.714,2.274,P﹥005)。[结论]在本实验剂量下未观察到氟铃脲对骨髓细胞和生殖细胞染色体结构畸变的诱发作用。
[Objective] To understand the genotoxic effect of hexaflumuron. [Method] 55 mice were selected and randomly divided into experimental group, negative control group and positive control group. The experimental group with different LD50 dose of hexaflumuron oral administration of mice by oral gavage, the negative control group with 5g / L sodium carboxymethyl cellulose solution by mouth, the positive control group 40mg / kg dose of intraperitoneal injection of phosphoramidite . Six hours after the last exposure to cervical dislocation, animals were sacrificed and bone marrow cells and primary spermatocyte chromosomes were prepared according to conventional methods. Chromosome aberrations of mouse bone marrow cells and testicular primary spermatocytes were analyzed. [Results] There were no significant differences in chromosome aberration rates between bone marrow cells and primary spermatocytes of all mice treated with hexaflumuron compared with the negative control group (χ2 = 2.285,0.502,0.144,0.198,0.159,0.471, 1.003, P> 005). There was no significant difference in the incidence of autosomal and sex chromosome premature separation between the primary spermatocytes in each dose exposure group and the negative control group (χ2 = 1.672,0.714,2.274, P> 005) . [Conclusion] No induction effect of hexaflumuron on chromosomal structural aberrations of bone marrow cells and germ cells was observed in this experiment.