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研究用1例人卵巢癌淋巴转移的癌组织直接移植于裸鼠皮下,建成一株人卵巢癌移植瘤动物模型,已传至第26代.移植成功率达100%,平均裸鼠带瘤存活中位数为102天.肿瘤倍增时间为7.17(SD=±1.02天).组织学和超微结构形态证实保持了原人肿瘤的特征,有淋巴结转移行为.人类肿瘤染色体特征.保留了分泌癌胚抗原的能力.具有P53癌基因蛋白的异常表达.移植瘤细胞可在体外培养并传至5代.流式细胞仪及显微分光光度计检测移植瘤,提示肿瘤为多倍体,各代移植瘤的DNA指数与第一代肿瘤基本一致(DI1.05~1.40).经初步应用研究,用传代移植瘤组织提取抗原免疫BALB/C小鼠,将免疫小鼠的脾细胞与小鼠缺欠型骨髓瘤系进行融合,制备了一株抗卵巢癌单克隆抗体,经免疫组化染色(ABC法)可显示有与人卵巢癌阳性反应.说明裸鼠人体卵巢癌模型的建立,为深入进行该类肿瘤诊断及治疗研究提供了有效途径.
In this study, a human ovarian cancer xenograft tumor model was established by subcutaneous transplantation of one human ovarian cancer lymphatic metastasis into nude mice, which has been transferred to the 26th generation.The successful rate of transplantation is 100%, and the average tumor survival in nude mice The median time to tumor doubling was 7.17 (SD = ± 1.02 days). Histological and ultrastructural features confirmed the characteristics of the primary human tumor with lymph node metastasis. Human tumor chromosomal characteristics. Embryonic antigen with the P53 oncoprotein abnormal expression of transplanted tumor cells can be cultured in vitro and passed to 5. Flow cytometry and micro-spectrophotometer detection of xenografts, suggesting that the tumor was polyploid, each generation Transplanted tumor DNA index and the first generation of tumors is basically the same (DI1.05 ~ 1.40) .According to the preliminary application of research, the BALB / C mice were immunized with the transplanted tumor tissue antigen, the immunized mice spleen cells and mouse defects Type myeloma line, a monoclonal antibody against ovarian cancer was prepared, which showed positive reaction with human ovarian cancer by immunohistochemical staining (ABC method) .To establish the model of human ovarian cancer in nude mice, This type of cancer diagnosis and treatment research provided Effective Ways.