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目的 探讨p5 3和 p6 3蛋白在肺癌中的表达及意义 ,并比较两者之间的表达关系。 方法 组织微阵 (tissuemicroarray ,TMA)技术和免疫组化S P方法 (immunohistochemistry ,IHC)。 结果 p5 3蛋白IHC染色中 ,TMA有价值的肿瘤标本为 2 34(72 .9% ) ,总的表达率 (+、++、+++)率为 4 8.3% (113/ 2 34)。随着肺鳞状细胞癌 (squamouscellcarcinoma,SCC)和腺癌(adenocarcinoma ,Ad)分级升高 ,p5 3蛋白表达增强 ,与肺SCC及Ad病理分级有明显相关性 (P <0 .0 5 )。p5 3蛋白表达与生存期呈明显负相关性 (P <0 .0 5 )。p5 3蛋白表达与病理分型和临床分期等无明显相关性 (P >0 .0 5 )。p5 3蛋白表达在肺癌原发肿瘤和转移淋巴结之间无明显差异 (P >0 .0 5 )。p6 3蛋白IHC染色中 ,TMA有价值的肿瘤标本为 2 6 4 (82 .2 % ) ,总的表达率(+、++、+++)为 6 7.8% (179/ 2 6 4 )。p6 3蛋白在小细胞肺癌 (smallcelllungcarcinoma,SCLC)表达最低 16 .0 % (4 / 2 5 ) ,非小细胞肺癌 (non smallcelllungcarcinoma ,NSCLC)与SCLC之间表达存在明显差异 (P <0 .0 5 )。p6 3蛋白表达与病理组织分级、临床分期、生存期等无明显相关性 (P >0 .0 5 )。p6 3蛋白表达在肺癌原发肿瘤和转移淋巴结之间无明显差异 (P >0 .0 5 )。结论 1.p5 3?
Objective To investigate the expression and significance of p5 3 and p6 3 protein in lung cancer and to compare their expressions. Methods Tissue microarray (TMA) and immunohistochemistry (IHC) were performed. Results In the IHC staining of p5 3 protein, TMA valuable tumor samples were 234 (72.9%) and the total expression rates (+, ++, +++) were 43.3% (113/234). With the increasing of grade of squamous cell carcinoma (SCC) and adenocarcinoma (Ad), the expression of p5 3 protein was increased, which was significantly correlated with the pathological grades of lung SCC and Ad (P <0.05). There was a negative correlation between p5 3 protein expression and survival (P <0.05). There was no significant correlation between p5-3 protein expression and pathological classification and clinical stage (P> 0.05). There was no significant difference in p5-3 protein expression between primary tumor and metastatic lymph node (P> 0.05). In the IHC staining of p63 protein, TMA valuable tumor samples were 26.4% (82.2%) with a total expression rate of (+, +++ +++) of 6 7.8% (179/264). There was a significant difference in the expression of p63 protein among SCLC (16.0%), NSCLC (SCLC) and non-small cell lung cancer (SCLC) (P <0.05) ). There was no significant correlation between p63 protein expression and histopathological grade, clinical stage and survival time (P> 0.05). The expression of p63 protein had no significant difference between primary tumor and metastatic lymph node (P> 0.05). Conclusion 1.p5 3?