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目的探讨紫杉醇对正常和乏氧状态下人宫颈癌细胞Hela的放射增敏作用。方法细胞乏氧模型、细胞放疗、采用四甲基偶氮唑蓝(MTT)快速比色法检测细胞增殖率;结果不同浓度的紫杉醇作用于乏氧和正常状态下的Hela细胞,均表现出增殖抑制作用,且呈浓度依赖性,乏氧状态下的Hela细胞增殖率明显大于正常状态下的细胞,两者相比差异有统计学意义(P<0.05),说明乏氧细胞可对对化疗药物表现出一定的抵抗作用。小剂量的紫杉醇(1×10-3mg/L)可增加正常和乏氧状态下的He-la细胞对放射线的敏感性,其增殖率分别为(19.67±5.06)%、(12.38±3.55)%,且两者相比差异无统计学意义(P>0.05)。结论乏氧细胞对化疗药物有一定的抵抗作用;紫杉醇对乏氧及正常状态下的Hela细胞均有抑制作用,且呈现浓度依赖性;小剂量的紫杉醇对乏氧状态下的Hela细胞具有放射增敏作用。
Objective To investigate the radiosensitization effect of paclitaxel on human cervical carcinoma Hela cells under normal and hypoxia conditions. Methods Cell hypoxia model and cell radiotherapy were used to detect cell proliferation rate by MTT colorimetric assay. Results Paclitaxel showed synergistic effects on proliferation of Hela cells under hypoxic and normal conditions Inhibitory effect in a dose-dependent manner. The proliferation rate of Hela cells in hypoxia was significantly higher than that in normal cells (P <0.05), indicating that hypoxic cells could inhibit the chemotherapeutic drugs Show some resistance. Small doses of paclitaxel (1 × 10-3mg / L) increased the sensitivity of He-la cells to radiation in normal and hypoxia conditions, with the proliferation rates of (19.67 ± 5.06)% and (12.38 ± 3.55)%, respectively , And there was no significant difference between them (P> 0.05). Conclusions The hypoxic cells have a certain resistance to chemotherapeutic drugs. Paclitaxel has an inhibitory effect on Hela cells under hypoxia and normal conditions, and in a concentration-dependent manner. Small doses of paclitaxel have a significant effect on Hela cells in hypoxia Sensitive effect.