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为探究毛白杨开花调控分子机制并获得不飞絮毛白杨株系,利用农杆菌介导法将显性负突变结构基因AP1M2转入毛白杨中,经PCR检测,共获得阳性转化植株7株。通过RT-qPCR检测转基因植株中外源基因AP1M2表达量,发现各株系间的表达水平差异显著,最高为内参的5.41倍,最低为1.89倍。对转基因毛白杨中内源PtAP1和其他开花关键基因的表达量进行检测,发现内源AP1的表达受到抑制,表达量明显下调;其他开花关键基因LFY、AP3、PI、SEP3和FT1等的表达量也有不同程度的降低,而FT2表达量并没有明显变化。这些研究结果表明转AP1M2毛白杨中AP1、LFY、AP3、PI、SEP3和FT1的表达受到明显抑制,对毛白杨花发育将有一定影响。
In order to explore the flowering and molecular mechanism of Populus tomentosa and obtain the Populus tomentosa-free poplar system, the dominant negative mutant AP1M2 gene was transferred into Populus tomentosa by Agrobacterium-mediated transformation. Seven positive transgenic plants were obtained by PCR. The expression level of exogenous gene AP1M2 in transgenic plants was detected by RT-qPCR. The expression level of AP1M2 in each line was significantly different, the highest was 5.41-fold and the lowest was 1.89-fold. The expression of endogenous PtAP1 and other flowering-related genes in transgenic Populus tomentosa was detected and found that the expression of endogenous AP1 was inhibited and the expression level was significantly down-regulated. The expression levels of other key flowering genes, LFY, AP3, PI, SEP3 and FT1 There are also different degrees of reduction, while FT2 expression did not change significantly. These results indicated that the expression of AP1, LFY, AP3, PI, SEP3 and FT1 in AP1M2-infected Populus tomentosa was significantly inhibited, which would have an impact on the development of Populus tomentosa.