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目的了解河南省2009年肠道病毒71型(EV71)流行株VP1基因特征。方法采用RT-PCR方法从手足口病患者粪便、咽拭子样品中扩增VP1区全长基因和序列测定,利用生物信息学软件对序列进行分析,构建序列遗传发育树。结果 262份样品中,VP1基因RT-PCR扩增鉴定结果显示111份样品阳性,选取其中20份样品进行VP1基因全序列测定,结果显示其与C4亚型代表株核苷酸同源性为90.8%～93.6%,氨基酸同源性为94.0%～99.3%。结论河南省手足口病患者感染EV71病毒的流行株属C基因型的C4亚型。
Objective To understand the characteristics of VP1 gene of enterovirus 71 (EV71) strain in Henan Province in 2009. Methods RT-PCR was used to amplify the full-length VP1 gene and its sequence in excrement and throat swab samples of HFMD patients. Bioinformatics software was used to analyze the sequence of the VP1 gene and establish a sequence-based genetic tree. Results Among the 262 samples, RT-PCR amplification of VP1 gene showed 111 positive samples. Twenty of the samples were selected for VP1 gene sequence analysis. The results showed that the nucleotide sequence of the VP1 gene was 90.8 % ~ 93.6%, amino acid homology was 94.0% ~ 99.3%. Conclusion The prevalence of EV71 virus in hand, foot and mouth disease patients in Henan Province is a C4 subtype of C genotype.