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目的 :探讨抗 HCV阴性献血员隐匿性HCV感染情况。方法 :采用逆转录套式PCR检测 10 0例抗HCV阴性献血员血清和外周血单个核细胞 (PBMC)中HCV RNA。结果 :血清中HCV RNA阳性 2例 ,PBMC中HCV RNA阳性 6例 ;有 1例血清及PBMC中HCV RNA均阳性。结论 :抗 HCV阴性的献血员中仍有少数存在HCV输血传播的可能 ,对献血员仅筛查抗 HCV是不够的 ,应对血清和PBMC中HCV RNA筛查
Objective: To investigate the occult HCV infection in anti-HCV negative blood donors. Methods: HCV RNA in serum and peripheral blood mononuclear cells (PBMCs) from 100 anti-HCV negative donors was detected by reverse transcription polymerase chain reaction (PCR). Results: Serum HCV RNA positive in 2 cases, PBMC HCV RNA positive in 6 cases; 1 cases of serum and PBMC HCV RNA were positive. CONCLUSIONS: Few anti-HCV negative blood donors still have the possibility of transmission of HCV blood transfusions. Screening anti-HCV only for blood donors is not enough, and screening of HCV RNA in serum and PBMC